【Detection principle】 This product uses fluorescence immunochromatography to quantitatively detect the content of glycocholic acid (CG) in dog/cat blood. Basic principle: T and C lines are drawn on the nitrocellulose membrane respectively, and the T line is coated with antigen a that specifically recognizes the antibody. The binding pad is sprayed with fluorescent nanomaterial-labeled antibody b that can specifically recognize antigen a. The antigen in the sample first combines with the nanomaterial-labeled antibody b to form a complex, and then goes up the chromatography. The more antigens in the sample bound by the complex The more, the less the fluorescent antibody combined with the T line, when the excitation light is irradiated, the nanomaterial emits a fluorescent signal, and the strength of the signal is inversely proportional to the antigen concentration in the sample.
【Detection purpose】 Cholyglycine (CG) is one of the conjugated cholic acids formed by the combination of bile acid and glycine. Glycocholic acid is the most important bile acid component in the serum of late pregnancy. When the liver cells are damaged, the ability of the liver cells to absorb CG decreases, resulting in an increase in the content of CG in the blood. In cholestasis, the hepatic excretion of bile acid is hindered, and the CG content of the reflux blood circulation increases, which also increases the blood CG content.
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